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Western blot analysis of vector particles encoding CD19-CAR (CD8-LV, CD4-LV or VSV LV) or GFP (CD8- -LVGFP) as transgene. 3.54x108 particles were separated on a 12% SDS polyacrylamide gel before transferred to a being PVDF membrane. Detection was performed by incubation of the membrane with an antibody specific for the
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Product Quantity Cat. No. Research-grade kit LV-MAX Lentiviral Production System Starter Kit For 0.3 L of LV vector production A35684 Research-grade cells Gibco Viral Production Cells . LV-MAX Transfection Kit For 1 L of LV vector production A35348 Opti-MEM I Reduced Serum Medium 100 mL 31985062 500 mL 31985070 10 x 500 mL 31985088
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